Chapter 6 Run shiny app
6.2 scRNA-seq
The scRNA-seq of one APL (AML with PML::RARA fusion, also called acute promyelocytic leukemia)) patient (including 3,000 cells) was used for illustration. The input format for RNA-seq analysis was a Seurat object.
The expample data of APL scRNA-seq (Seurat object) can be downloaded APL scrna seurat demo data or generated use Seurat::CreateSeuratObject.
Click Upload to upload the seurat object.
And wait for about one minute for calculation.
Visualization of cosine-like score of the subcluster.
Circle visualization of APL, colored by cell type.
Circle visualization of APL, colored by cell proportion.
Cluster-tree visualization of APL, absolute proportional mode.
Cluster-tree visualization of APL, relative proportional mode.
Cluster-tree visualization of the reference, relative proportional mode.
6.3 Bulk RNA-seq
The expample data of B-ALL bulk RNA-seq can be downloaded via B-ALL bulk demo data
The expample data of AML bulk RNA-seq can be downloaded via AML bulk demo data
Click Upload to upload the expression matrix.
Cluster-tree visualization of B-ALL (colored by score value).
Cluster-tree visualization of B-ALL (colored by cell type).
Lollipop visualization of AML (colored by cell type).
